OncoGen is a research centre for Gene and Cellular Therapies in Cancer

Applicability of research results in clinical practice

The first research centre in Romania and Eastern Europe, with state-of-the-art equipment for basic and applied research in the field of gene therapies. The Centre has basic and applied research facilities, with 15 compartments including a GMP facility and laboratories endowed with high performance equipment.


The main research projects developed at OncoGen

Cellular and gene therapies for cancer

Chimeric antigen receptor targeted oncoimmunotherapy with NK cells

Molecular approach to ragweed allergy diagnosis and therapy

Romania-Serbia Joint Initiative against Cancer in Cross-Border Region: Improved Diagnosis and Treatment of Malignant Tumors

BIOeconomic approach to AntiMicrobial agents – use and Resistance

BIOnotechnologies based on Extracellular Vesicles for early diagnosis, prognosis and therapy of atherosclerosis


OncoGen’s research activities at a glance

“Diaspora in Scientific Research and Higher Education in Romania – Diaspora and friends” Conference

“Diaspora in Scientific Research and Higher Education in Romania – Diaspora and friends” Conference

OncoGen News Scientific Events
Keeping the tradition of organizing the conference Romanian scientific Diaspora, the Executive Unit for Financing Higher Education, Research, Development and...
Read More
Award for OncoGen Researcher at the European Society of Cardiology Meeting in Malaga

Award for OncoGen Researcher at the European Society of Cardiology Meeting in Malaga

OncoGen Researchers
Dr. Greta Bărbulescu presented at the International Conference organized by the European Society of Cardiology (ESC) Working Group – Development,...
Read More
1 2 3
Alergia la ambrozia. Fapte

Alergia la ambrozia. Fapte

Echipa proiectului INSPIRED a Centrului de Cercetare OncoGen Timișoara sprijină Consiliului Județean Timiș  în activitatea de combatere a ambroziei din cadrul proiectului STOP AMBROZIA – Program OcroTim. CJ Timiș a lansat pentru cetățenii județului Timiș o aplicație de identificare și localizare a buruienii ( cu scopul  înlăturării ambroziei din locațiile semnalate de cetățeni.

De asemenea, persoanele alergice la ambrozia pot afla momentul începerii sezonului polinic și nivelul alertei alergice sezoniere, accesând link-ul :

Totodată, coordonatorul proiectului INSPIRED, prof. univ. dr. Carmen Panaitescu Bunu, medic primar alergolog, vine în sprijinul persoanelor alergice la ambrozia oferind răspunsuri la întrebările legate de manifestările alergice după completarea unui mini-chestionar ce cuprinde evaluarea simptomelor:

OncoGen COVID-19 vaccine project recognized by the World Health Organization (WHO)

OncoGen COVID-19 vaccine project recognized by the World Health Organization (WHO)

The World Health Organization (WHO) recognized OncoGen, as the only research centre in Romania that has a protein subunit COVID-19 vaccine candidate in pre-clinical tests.

OncoGen joins reputable academic institutions and private companies known for their expertise in the field of vaccine development and high-performing biomedical research.

The full list of candidate vaccines in clinical and pre-clinical tests, as of April 4, 2020, can be found at:

OncoGen researchers  propose personalized vaccinomics strategy for the novel China coronavirus

OncoGen researchers propose personalized vaccinomics strategy for the novel China coronavirus

OncoGen researchers propose a novel approach for producing epitope-based peptide vaccine candidates for the novel China coronavirus (nCoV).

Wuhan seafood market pneumonia virus, also known as 2019 Novel Coronavirus (2019-nCoV) is a relative of both the deadly severe acute respiratory syndrome (SARS) and the Middle East respiratory syndrome (MERS) viruses. According to Nature Briefing, by January 28, the number of confirmed cases has jumped to more than 4,500 with more than 100 people dead as a result of the infection. By comparison, SARS spread globally in 2002-03, infecting 8000 and killing more than 700 people.

Traditional vaccines for viral diseases had limited success in the case of viral epidemics with high mutation rates. The approach that we used for designing potential peptide vaccine candidates takes a more personalized strategy, referred to as vaccinomics, which enables the selection of target viral proteins with low mutation rates, and the design of peptides tuned to the immune characteristics of the target populations, variable based on geographic location and ethnicity.

Based on the human MHC (human leukocyte antigen) allele variation, the peptide vaccines can be made more relevant to a community or individual. The selection of the peptides that could act as vaccines is determined by the binding of the processed viral peptide with the major histocompatibility complex (MHC) class I and II molecules, and the relevant HLA alleles.

Our proposed candidate peptides take into consideration the most frequent HLA alleles found in the Romanian population.

Technical details of the approach used are detailed below:

We identified multiepitope peptide vaccine candidates against nCov that can potentially trigger both CD4+ and CD8+ T cell immune response. To this end, we designed 15-30 aa synthetic long peptides (SLPs), as proposed by Rabu et al. (2019), using a cathepsin-sensitive linker (LLSVGG) for linking MHC class I-restricted epitopes to MHC class II-restricted epitopes, with the MHC class II epitope located always at the N-terminal end, to stimulate both CTLs and T helper lymphocytes (together with innate immune system).

GenBank Acc. No. of coronavirus isolate Wuhan-Hu-1 used for analysis: MN908947.3 which is identical to Ref Seq NC_045512 (provisonal ref seq, under review by NCBI):

It is an enveloped, positive-sense, single-stranded poly-adenylated RNA virus, with a genome of 29.9 kb (29903 bp), among the largest of all RNA virus genomes.

Complete genomic sequences known to date: S and M proteins important as target for nCoV vaccine; S = spike (for adhesion and fusion); M = membrane (for replication and virion exocytosis).

The tool from the Immune Epitope Database (IEDB) server was used to identify potent T cell epitopes from the sequences of S and M proteins. For CD8+ T cell epitopes, the prediction method integrated MHC class I binding, proteasomal cleavage and TAP transport efficiency. Several epitopes were selected for further analysis based on high overall score and if they showed high MHC binding affinity (IC50 <50 nM). For CD4+ T cell epitopes, the highest ranking peptide based on MHC class II binding was chosen.

The list of selected T cell epitopes based on the most frequent class I and class II MHC alleles in the Romanian population (taken as an example to demonstrate a proof-of-concept) are found in our Preprints manuscript.

The candidate peptides were designed by applying to viral antigens a technology used for personalized vaccination in cancer, based on long neoantigen peptides, currently in clinical trials for cancer therapy.
Based on this model, additional peptide candidates can be identified for populations from different geographic regions of the world.
The current approach is a compromise between an industrial large-scale global vaccination strategy and an individually-targeted personalized vaccination strategy.
We will continue the validation of these candidates and of the model.

Image source: Eye of Science/SPL


Pius Branzeu Clinical Emergency Hospital
300723 Timisoara, Romania
156, Liviu Rebreanu Boulevard

© OncoGen Research Centre. 2019-2020. All rights reserved. Website by Ecuson Studio