Development of software packages for programming bioprinters in three-dimensional bioprinting

Details:

• Development of software packages for printing tumor models (toroidal and triple layered). The programs were written in Python, have a graphical interface, and allow the generation of G-code instructions with scalable geometry.
• Experimental validation of the developed programs. Both types of structures, containing tumor and peritumoral cells, were printed individually or consecutively in multiwell plates.
• Evaluation of printed constructs. In vitro evolution of the printed constructs was assessed for cell viability and proliferation.

Publications:

1. Bojin F, Robu A, Bejenariu MI, Ordodi V, Olteanu E, Cean A, Popescu R, Neagu M, Gavriliuc O, Neagu A, Paunescu V, 3D Bioprinting of Model Tissues That Mimic the Tumor Microenvironment. Micromachines 2021, 12, 535. https://doi.org/10.3390/mi12050535
2. Arjoca S, Robu A, Neagu M, Neagu A, Mathematical and computational models in spheroid-based biofabrication. Acta Biomaterialia 2023, 165, 125-139. https://doi.org/10.1016/j.actbio.2022.07.024

Participation in conferences:

1. Bojin F, Robu A, Bejenariu MI, Ordodi V, Olteanu E, Cean A, Popescu R, Neagu M, Gavriliuc O, Neagu A, Arjoca S, Paunescu V. "3D bioprinting of model tissues that mimic the tumor microenvironment", poster , 13th European Biophysics Conference (EBSA 2021), 24-28 July 2021, Vienna, Austria
2. Bojin F, Robu A, Bejenariu MI, Ordodi V, Olteanu E, Cean A, Popescu R, Neagu M, Gavriliuc O, Neagu A, Arjoca S, Paunescu V. "3D bioprinting of model tissues that mimic the tumor microenvironment", poster , International Conference on Biofabrication, September 27-29, 2021, online event, Wollongong, Australia
3. Bojin F, Robu A, Bejenariu MI, Ordodi V, Olteanu E, Cean A, Popescu R, Neagu M, Gavriliuc O, Neagu A, Arjoca S, Paunescu V. "3D bioprinting of model tissues that mimic the tumor microenvironment", poster , 1st Oncohub Conference, October 13-15, 2021, online event, Bucharest, Romania
4. Bojin F, Arjoca S, Bejenariu MI, Ordodi V, Olteanu E, Cean A, Popescu R, Neagu M, Gavriliuc O, Neagu A, Paunescu V. "Biofabtication of tumor models that mimic the tumor microenvironment using extrusion bioprinting" - oral presentation , Tissue Engineering and Regenerative Medicine International Society (TERMIS) European Conference 2022, June 26 – July 1, 2022, Krakow, Poland
5. Arjoca S, Robu A, Bojin F, Ordodi V, Olteanu E, Cean A, Neagu M, Gavriliuc O, Neagu A, Paunescu V. "Post-printing structure formation in bioprinted tissue constructs that mimic the tumor microenvironment" - oral presentation, Tissue Engineering and Regenerative Medicine International Society (TERMIS) European Conference 2022, June 26 – July 1, 2022, Krakow, Poland
6. Bojin F, Arjoca S, Bejenariu MI, Ordodi V, Olteanu E, Cean A, Popescu R, Neagu M, Gavriliuc O, Neagu A, Paunescu V. "Biofabrication of breast cancer models using extrusion bioprinting" - oral presentation, 17th National Conference of Biophysics with International Participation (CNB2022), September 23-25, 2022, Târgu Mureș, Romania
7. Arjoca S, Robu A, Bojin F, Ordodi V, Olteanu E, Cean A, Neagu M, Gavriliuc O, Neagu A, Paunescu V. "Multicellular self-organization in bioprinted models of tumor microenvironment" - oral presentation, 17th National Conference of Biophysics with International Participation (CNB2022), September 23-25, 2022, Târgu Mureș, Romania

Details:

• Development of software packages for bioprinting of blood vessels. The Python language was used for the development of the software and the graphical interface. The targeted geometry is helical tube and can be easily scaled.
• Experimental validation of the developed programs. Blood vessel patterns of various sizes have been successfully printed individually or in multi-well plates.
• Evaluation of post-printing constructs. The blood vessel models were histologically evaluated at different time periods of in vitro culture.

Publications: manuscript in progress for completion and publication.

Participation in conferences:

1. Nistor A, Arjoca S, Bojin F, Zoric A, Salagean A, Paunescu V. "Bioengineering of implantable 3D vascular bioconstruct using rapid prototyping techniques", poster, 1st Oncohub Conference, October 13-15, 2021, online event, Bucharest, Romania

Details:

• Development of Python scripts for generating and printing complex 3D geometries.
• Testing the printability of some hydrogels using the developed models. The constructs were analyzed from the point of view of reproducibility and geometric (shape fidelity, deflection of suspended filaments, etc.) or process (extrusion speed, applied pressure, diameter of deposited filament, etc.) factors.

Publications: manuscript in progress for completion and publication.