Short description:

In the genomics laboratory, we investigate the genetic and epigenetic variations associated with the susceptibility to the development of some forms of cancer in order to use the information obtained for better diagnostic and prognostic methods. The laboratory is equipped with versatile automation systems with a wide range of possible scientific applications: extraction of genetic material and analysis of a comprehensive panel of under- or over-expressed genes in cancer, identification of point mutations (SNPs) or other genetic variations by DNA sequencing, analysis of gene methylation status by bisulfite treatment method and DNA sequencing. In collaboration with the Microarray and Cell Culture Laboratories of the OncoGen Institute, genes associated with cancer susceptibility identified by screening are validated and confirmed by in vitro tests on human tumor cell lines.

Main activities/techniques:

• Isolation of genetic material from different types of cell cultures, as well as patient samples (DNA, RNA, microRNA, etc.)
• Gene expression analysis
• Gene methylation status analysis
• Amplicon DNA sequencing
• SNP genotyping

Personal:

• Coordinator: Oana Gavriliuc
• Florina Bojin
• Ada Cean
• Alexandra Ivan
• Laboratory technician: Marieta Neata

Equipment:

• MagMAX Express 96 Nucleic Acid Extractor (ThermoFisher)
• Real-time PCR system (Roche Lightcycler 480 II)
• Hazard Sequencing System
• Genetic Analyzer 3130XL Sequencer (AB / Hitachi)