[et_pb_section fb_built=”1″ _builder_version=”4.22.1″ _module_preset=”default” custom_padding=”0px|0px|0px|0px|true|true” global_colors_info=”{}”][et_pb_row column_structure=”3_5,2_5″ use_custom_gutter=”on” gutter_width=”2″ make_equal=”on” _builder_version=”4.22.1″ _module_preset=”default” width=”100%” max_width=”100%” global_colors_info=”{}”][et_pb_column type=”3_5″ _builder_version=”4.22.1″ _module_preset=”default” background_enable_image=”off” background_size=”contain” global_colors_info=”{}”][et_pb_image src=”https:\/\/oncogen.oncoserv.ro\/wp-content\/uploads\/2023\/09\/nanocel5.jpg” title_text=”nanocel5″ _builder_version=”4.22.1″ _module_preset=”default” hover_enabled=”0″ border_radii=”on|10px|10px|10px|10px” global_colors_info=”{}” align=”center” force_fullwidth=”on” sticky_enabled=”0″][\/et_pb_image][\/et_pb_column][et_pb_column type=”2_5″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_blurb title=” \/\/ Proiect ” content_max_width=”100%” _builder_version=”4.22.1″ _module_preset=”default” header_font=”|600||on|||||” header_font_size=”20px” body_font=”|700||on|||||” body_text_color=”#000000″ body_font_size=”40px” header_font_size_tablet=”” header_font_size_phone=”18px” header_font_size_last_edited=”on|phone” body_font_size_tablet=”” body_font_size_phone=”29px” body_font_size_last_edited=”on|phone” custom_css_blurb_title=”color:var(–color-1);padding-bottom:15px;” custom_css_blurb_content=”line-height:45px;color:var(–color-2);” global_colors_info=”{}” custom_css_blurb_content_last_edited=”on|phone” custom_css_blurb_content_phone=”line-height:39px;color:var(–color-2);” custom_css_blurb_content_tablet=”line-height:45px;color:var(–color-2);”]<\/p>\n
Proiectul NANOCEL<\/p>\n
[\/et_pb_blurb][et_pb_blurb title=”Perioada de desf\u0103\u0219urare” content_max_width=”100%” module_class=”dct_ptf_dt” _builder_version=”4.22.1″ _module_preset=”default” header_font=”|700||on|||||” header_font_size=”20px” body_font=”|600|||||||” body_font_size=”17px” global_colors_info=”{}”]<\/p>\n
2016 \u2013 2017<\/span><\/p>\n [\/et_pb_blurb][et_pb_blurb title=”Nr. proiect” content_max_width=”100%” module_class=”dct_ptf_dt” _builder_version=”4.22.1″ _module_preset=”default” header_font=”|700||on|||||” header_font_size=”20px” body_font=”|600|||||||” body_font_size=”17px” global_colors_info=”{}”]<\/p>\n \u00a0PIII-C4-PCFI-2016\/2017-03<\/span><\/p>\n [\/et_pb_blurb][et_pb_blurb title=”Coordonator” content_max_width=”100%” module_class=”dct_ptf_dt” _builder_version=”4.22.1″ _module_preset=”default” header_font=”|700||on|||||” header_font_size=”20px” body_font=”|600|||||||” body_font_size=”17px” global_colors_info=”{}”]<\/p>\n Universitatea de Medicin\u0103 \u0219i Farmacie \u201dVictor Babe\u0219\u201d din Timi\u0219oara<\/span><\/p>\n [\/et_pb_blurb][et_pb_blurb title=”Director proiect” content_max_width=”100%” module_class=”dct_ptf_dt” _builder_version=”4.22.1″ _module_preset=”default” header_font=”|700||on|||||” header_font_size=”20px” body_font=”|600|||||||” body_font_size=”17px” custom_margin=”||40px||false|false” global_colors_info=”{}”]<\/p>\n Prof. Dr. Virgil P\u0103unescu<\/span><\/p>\n [\/et_pb_blurb][et_pb_blurb title=”Finan\u021bare” content_max_width=”100%” module_class=”dct_ptf_dt” _builder_version=”4.22.1″ _module_preset=”default” header_font=”|700||on|||||” header_font_size=”20px” body_font=”|600|||||||” body_font_size=”17px” custom_margin=”||40px||false|false” global_colors_info=”{}”]<\/p>\n 135.000 RON<\/p>\n [\/et_pb_blurb][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.16″ _module_preset=”default” width=”100%” max_width=”100%” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_blurb title=”Obiective generale:” content_max_width=”100%” _builder_version=”4.22.1″ _module_preset=”default” header_font=”|700||on|||||” header_font_size=”26px” body_font_size=”17px” body_line_height=”29px” global_colors_info=”{}”]<\/p>\n (1)\u00a0<\/strong>identificarea mecanismelor de moarte celular\u0103 \u00een prezen\u021ba nanoparticulelor de Fe3O4;\u00a0<\/span><\/p>\n (2)<\/strong>\u00a0evaluarea influen\u021bei acestor nanoparticule \u00een formarea \u0219i dezvoltarea tumorilor.<\/span><\/p>\n [\/et_pb_blurb][et_pb_blurb title=”Obiective specifice:” content_max_width=”100%” _builder_version=”4.22.1″ _module_preset=”default” header_font=”|700||on|||||” header_font_size=”26px” body_font_size=”17px” body_line_height=”29px” global_colors_info=”{}”]<\/p>\n (1)<\/strong>\u00a0Caracterizarea morfologic\u0103, fenotipic\u0103, genotipic\u0103 \u0219i func\u021bional\u0103\u00a0<\/span>in vitro<\/em>\u00a0a tumorilor solide \u00een prezen\u021ba nanoparticulelor de magnetit\u0103 (Fe3O4);\u00a0<\/span><\/p>\n (2)<\/strong>\u00a0Evaluarea mecanismelor de moarte celular\u0103 indus\u0103 de nanoparticule;\u00a0<\/span><\/p>\n (3)<\/strong>\u00a0Identificarea\u00a0<\/span>in vitro<\/em>\u00a0a markerilor caracteristici rezulta\u021bi din interac\u021biunea celulelor tumorale expuse la nanoparticule cu micromediul tumoral;\u00a0<\/span><\/p>\n (4)<\/strong>\u00a0Evaluarea\u00a0<\/span>in vivo<\/em>\u00a0a capacit\u0103\u021bi celulelor tumorale expuse la nanoparticule de a forma \u0219i dezvolta tumori<\/span><\/p>\n [\/et_pb_blurb][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.16″ _module_preset=”default” width=”100%” max_width=”100%” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_blurb title=”Rezumatul proiectului” content_max_width=”100%” _builder_version=”4.22.1″ _module_preset=”default” header_font=”|700||on|||||” header_font_size=”26px” body_font_size=”17px” body_line_height=”29px” global_colors_info=”{}”]<\/p>\n \u00cen timpul procesului de tumorigenez\u0103, celulele tumorale prolifereaz\u0103 necontrolat, elud\u00e2nd mecanismele reglatoare ale mor\u021bii celulare. Au fost descrise mai multe tipuri de moarte celular\u0103: apoptoza (tip I), moarte celular\u0103 asociat\u0103 autofagiei (tip II), necroza (tip III), catastrofa mitotic\u0103, mecanisme dependente de ancorare \u2013 anoikis, excitotoxicitatea, degenerescen\u021ba Wallerian\u0103, cornificarea pielii. Acest proiect propune investigarea unui posibil nou mecanism de inducere a mor\u021bii celulare tumorale in vitro \u0219i in vivo \u2013 enucleerea. Se va testa influen\u021ba nanoparticulelor de Fe3O4 \u00een suspensii coloidale asupra liniilor celulare tumorale umane. Particulele magnetice (MNPs) vor fi sintetizate prin combustie \u0219i acoperite cu dublu strat de acid oleic, interac\u021bion\u00e2nd cu celulele tumorale. Studiile in vitro vor fi focusate pe modific\u0103rile morfologice \u0219i ultrastructurale, studii func\u021bionale, markeri imunofenotipici \u0219i expresia genic\u0103 induse la nivelul celulelor tumorale \u0219i nucleilor rezulta\u021bi, folosind o metodologie modern\u0103. Prin modelele in vivo va fi detectat\u0103 abilitatea celulelor tumorale tratate cu nanoparticule magnetice de a forma tumori la \u0219oareci imunodeficien\u021bi, urmat\u0103 de caracterizarea tumorilor generate \u0219i a metastazelor asociate. Acest proiect va aduce dovezi referitoare la enucleere ca mecanism poten\u021bial al mor\u021bii celulare tumorale, gener\u00e2nd noi direc\u021bii \u00een cercetarea biologiei tumorale \u0219i dezvoltarea de agen\u021bi terapeutici capabili s\u0103 exploateze acest comportament.<\/span><\/p>\n [\/et_pb_blurb][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.16″ _module_preset=”default” width=”100%” max_width=”100%” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_blurb title=” Rezultate” content_max_width=”100%” _builder_version=”4.22.1″ _module_preset=”default” header_font=”|700||on|||||” header_font_size=”26px” body_font_size=”17px” body_line_height=”29px” global_colors_info=”{}”]<\/p>\n [\/et_pb_blurb][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.16″ _module_preset=”default” width=”100%” max_width=”100%” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_blurb title=”Articole in extenso” content_max_width=”100%” _builder_version=”4.22.1″ _module_preset=”default” header_font=”|700||on|||||” header_font_size=”26px” body_font_size=”17px” body_line_height=”29px” global_colors_info=”{}”]<\/p>\n [\/et_pb_blurb][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_2,1_2″ _builder_version=”4.22.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”1_2″ _builder_version=”4.22.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_image src=”https:\/\/oncogen.ro\/wp-content\/uploads\/2023\/09\/nanocel1.jpg” title_text=”nanocel1″ align=”center” _builder_version=”4.22.1″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_image][et_pb_text _builder_version=”4.22.1″ _module_preset=”default” global_colors_info=”{}”]<\/p>\n Fig. 1. Imaginile SEM corespunz\u0103toare celulelor mezenchimale (MSC) \u015fi tumorale (SK-BR-3), \u00eenainte \u015fi dup\u0103 tratamentul cu suspensiile coloidale magnetice: A \u2013 celule mezenchimale (MSC) netratate cu suspensii coloidale magnetice (control); B \u2013 celule mezenchimale tratate cu suspensie coloidal\u0103 1F; C \u2013 celule mezenchimale tratate cu suspensie coloidal\u0103 2F; D \u2013 celule tumorale SK-BR-3 netratate cu suspensii coloidale magnetice (control); E \u2013 celule tumorale tratate cu suspensia coloidal\u0103 1F; F \u2013 celule tumorale tratate cu suspensia coloidal\u0103 2F.<\/span><\/p>\n [\/et_pb_text][et_pb_image src=”https:\/\/oncogen.ro\/wp-content\/uploads\/2023\/09\/nanocel3.jpg” title_text=”nanocel3″ align=”center” _builder_version=”4.22.1″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_image][et_pb_text _builder_version=”4.22.1″ _module_preset=”default” global_colors_info=”{}”]<\/p>\n Fig.3. Imaginile TEM corespunz\u0103toare celulelor tumorale SK-BR-3, \u00een urma trat\u0103rii cu suspensii coloidale magnetice; A \u015fi B \u2013 celule tumorale tratate cu suspensia coloidal\u0103 2F (Fe3O4 \u2192 precipitare); C \u015fi D \u2013 celule tumorale tratate cu suspensia coloidal\u0103 1F (Fe3O4 \u2192 combustie).<\/span><\/p>\n [\/et_pb_text][et_pb_image src=”https:\/\/oncogen.ro\/wp-content\/uploads\/2023\/09\/nanocel5.jpg” title_text=”nanocel5″ align=”center” _builder_version=”4.22.1″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_image][et_pb_text _builder_version=”4.22.1″ _module_preset=”default” global_colors_info=”{}”]<\/p>\n Fig.5. Analiza imunocitochimic\u0103 a celulelor mezenchimale (MSC), respectiv tumorale (SK-BR-3) marcate cu protein\u0103 citoscheletic\u0103 Vimentin, respectiv cu oncoprotein\u0103 Her2; A \u2013 celule mezenchimale netratate (control); B \u2013 celule mezenchimale tratate cu suspensia coloidal\u0103 1F; C \u2013 celule mezenchimale tratate cu suspensia coloidal\u0103 2F; D \u2013 celule tumorale netratate (control); E \u2013 celule tumorale tratate cu suspensia coloidal\u0103 1F; F \u2013 celule tumorale tratate cu suspensia coloidal\u0103 2F.<\/span><\/p>\n [\/et_pb_text][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.22.1″ _module_preset=”default” global_colors_info=”{}”][et_pb_image src=”https:\/\/oncogen.ro\/wp-content\/uploads\/2023\/09\/nanocel2.jpg” title_text=”nanocel2″ align=”center” _builder_version=”4.22.1″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_image][et_pb_text _builder_version=”4.22.1″ _module_preset=”default” global_colors_info=”{}”]<\/p>\n Fig.2. Imaginile TEM corespunz\u0103toare celulelor netratate cu suspensii coloidale magnetice: A \u2013 celule mezenchimale aderente, cultivate pe inserturi celulare; B \u2013 celule mezenchimale (MSC) \u00een suspensie; C \u2013 celule tumorale (SK-BR-3) aderente, cultivate pe inserturi celulare; D \u2013 celule tumorale \u00een suspensie.<\/span><\/p>\n [\/et_pb_text][et_pb_image src=”https:\/\/oncogen.ro\/wp-content\/uploads\/2023\/09\/nanocel4.jpg” title_text=”nanocel4″ align=”center” _builder_version=”4.22.1″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_image][et_pb_text _builder_version=”4.22.1″ _module_preset=”default” global_colors_info=”{}”]<\/p>\n Fig. 4. Analiza flowcitometric\u0103 a celulelor mezenchimale (MSC) \u00eenainte \u015fi dup\u0103 tratarea cu suspensii coloidale magnetice: A, B \u015fi C \u2013 celule mezenchimale netratate (control); D, E \u015fi F \u2013 celule mezenchimale tratate cu suspensia coloidal\u0103 2F; G, H \u015fi I \u2013 celule mezenchimale tratate cu suspensia coloidal\u0103 1F.<\/span><\/p>\n [\/et_pb_text][et_pb_image src=”https:\/\/oncogen.ro\/wp-content\/uploads\/2023\/09\/nanocel6.jpg” title_text=”nanocel6″ align=”center” _builder_version=”4.22.1″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_image][et_pb_text _builder_version=”4.22.1″ _module_preset=”default” global_colors_info=”{}”]<\/p>\n Fig.6. Dezvoltarea tumoral\u0103 \u00een model animal de \u0219oarece imunosupresat CD1 Nu\/Nu<\/span><\/p>\n [\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":" Proiectul NANOCEL2016 \u2013 2017\u00a0PIII-C4-PCFI-2016\/2017-03Universitatea de Medicin\u0103 \u0219i Farmacie \u201dVictor Babe\u0219\u201d din Timi\u0219oaraProf. Dr. Virgil P\u0103unescu135.000 RON(1)\u00a0identificarea mecanismelor de moarte celular\u0103 \u00een prezen\u021ba nanoparticulelor de Fe3O4;\u00a0 (2)\u00a0evaluarea influen\u021bei acestor nanoparticule \u00een formarea \u0219i dezvoltarea tumorilor.(1)\u00a0Caracterizarea morfologic\u0103, fenotipic\u0103, genotipic\u0103 \u0219i func\u021bional\u0103\u00a0in vitro\u00a0a tumorilor solide \u00een prezen\u021ba nanoparticulelor de magnetit\u0103 (Fe3O4);\u00a0 (2)\u00a0Evaluarea mecanismelor de moarte celular\u0103 indus\u0103 de […]<\/p>\n","protected":false},"author":2,"featured_media":100880,"comment_status":"open","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","inline_featured_image":false,"footnotes":""},"project_category":[],"project_tag":[],"class_list":["post-100629","project","type-project","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/oncogen.ro\/ro\/wp-json\/wp\/v2\/project\/100629","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/oncogen.ro\/ro\/wp-json\/wp\/v2\/project"}],"about":[{"href":"https:\/\/oncogen.ro\/ro\/wp-json\/wp\/v2\/types\/project"}],"author":[{"embeddable":true,"href":"https:\/\/oncogen.ro\/ro\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/oncogen.ro\/ro\/wp-json\/wp\/v2\/comments?post=100629"}],"version-history":[{"count":6,"href":"https:\/\/oncogen.ro\/ro\/wp-json\/wp\/v2\/project\/100629\/revisions"}],"predecessor-version":[{"id":101016,"href":"https:\/\/oncogen.ro\/ro\/wp-json\/wp\/v2\/project\/100629\/revisions\/101016"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/oncogen.ro\/ro\/wp-json\/wp\/v2\/media\/100880"}],"wp:attachment":[{"href":"https:\/\/oncogen.ro\/ro\/wp-json\/wp\/v2\/media?parent=100629"}],"wp:term":[{"taxonomy":"project_category","embeddable":true,"href":"https:\/\/oncogen.ro\/ro\/wp-json\/wp\/v2\/project_category?post=100629"},{"taxonomy":"project_tag","embeddable":true,"href":"https:\/\/oncogen.ro\/ro\/wp-json\/wp\/v2\/project_tag?post=100629"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}\n
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