Genomics Department

Short description:

​In the genomics laboratory we investigate the genetic and epigenetic variations associated with the susceptibility of developing some forms of cancer in order to use the information obtained for better diagnostic and prognostic methods. The lab is equipped with versatile automation systems with a wide range of possible scientific applications: extraction of genetic material and analysis of a comprehensive panel of sub- or over-expressed genes in cancer, identification of point mutations (SNPs) or other genetic variations by DNA sequencing, analysis of gene methylation status by the bisulfite treatment method and DNA sequencing. In collaboration with the Microarray and Cell Culture Laboratories of the OncoGen Institute, genes associated with cancer susceptibility identified by screening are further validated and confirmed by in vitro tests on human tumor cell lines.

​Main activities / techniques:

• ​isolation of genetic material from different types of cell cultures, as well as patient samples (DNA, RNA, microARN, etc.)
• gene expression analysis
• gene methylation status analysis
• amplicon DNA sequencing
• genotyping of SNP

Staff:

• ​Coordinator: Oana Gavriliuc, MS, PhD
• Florina Bojin, MD, PhD
• Ada Cean, Biotech Eng., PhD
• Alexandra Ivan, Biotech Eng., PhD
• Technicians

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Equipment:

• ​MagMAX Express 96 Nucleic Acid Extractor (ThermoFisher)
• Real-time PCR system (Roche Lightcycler 480 II)
• Sanger sequencing system
• Genetic Analyzer 3130XL Sequencer (AB / Hitachi)