Cell Histology and Imaging Laboratory

Short description:

​The laboratory is dedicated to investigating biological and genetic aspects in cancer and other diseases, developing and applying top technology in histopathological diagnosis, providing collaborative support for clinical research protocols. The lab offers a wide range of histological research techniques including: standard paraffin inclusion methods, special tissue orientation, serial segmentation, and frozen sample separation. In addition to colouring with hematoxylin and eosin the paraffin sections, there is also special colouring to identify cells, other specific components of tissues or other cellular elements. The lab features advanced microscopic blade imaging equipment, as well as a telepathology photo system that allows tridimensional reconstitution of samples.

​​Main activities / techniques:

• ​preparation of histological sections of frozen paraffin-embedded tissues including serial sections
• hematoxylin-eosin colouring
• special colouring
• immunohistochemical coloring

Staff:

• ​Coordinator: Daciana Nistor, MD, PhD
• Calin Tatu, MD, PhD
• Alexandra Ivan, Biotech. Eng., PhD
• Technicians

Equipment:

• ​Carl Zeiss Confocal Microscope Axio Observer.Z1
• Carl Zeiss Axis Observer D1 inverse microscope
• Direct Carl Zeiss Axio Scope A1 Microscope
• Hund microscope with fluorescent lamp and camera
• Nikon E 600 direct light microscope with fluorescent lamp and camera
• Olympus inverted light microscope with digital capture and storage of microscopic images (Olympus camera, Olympus digital camera, VHS monitor, fluorescent lamp)
• Criotom Leica CM 1950
• Leica RM 2255 Microtome
• Carl Zeiss Axio Imager Z2 telepathology camera