- \n
- Dezvoltarea de pachete software pentru tip\u0103rirea de modele tumorale (toroidal \u0219i triplu stratificat).<\/em> Programele au fost scrise \u00een limbajul Python, au interfa\u021b\u0103 grafic\u0103, \u0219i permit generarea de instruc\u021biuni de tip G-code cu geometrie scalabil\u0103.<\/li>\n
- Validarea experimental\u0103 a programelor dezvoltate<\/em>. Ambele tipuri de structuri, con\u021bin\u00e2nd celule tumorale \u0219i peritumorale, au fost tip\u0103rite individual sau consecutiv \u00een pl\u0103ci cu mai multe godeuri.<\/li>\n
- Evaluarea constructelor tip\u0103rite<\/em>. Evolu\u021bia in vitro a constructelor tip\u0103rite a fost evaluat\u0103 din punct de vedere al viabilit\u0103\u021bi \u0219i prolifer\u0103rii celulare.<\/li>\n<\/ul>\n
![\"\"](\"https:\/\/oncogen.ro\/wp-content\/uploads\/2023\/12\/Figura_prototip_organoizi_tumorali1-300x193.png\")
\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0![\"\"](\"https:\/\/oncogen.ro\/wp-content\/uploads\/2023\/12\/Figura_prototip_organoizi_tumorali2-300x263.png\")
<\/p>\nPublica\u021bii<\/strong>:<\/p>\n
- \n
- Bojin F, Robu A, Bejenariu MI, Ordodi V, Olteanu E, Cean A, Popescu R, Neagu M, Gavriliuc O, Neagu A, Paunescu V, 3D Bioprinting of Model Tissues That Mimic the Tumor Microenvironment. Micromachines 2021, 12, 535. https:\/\/doi.org\/10.3390\/mi12050535<\/a><\/li>\n
- Arjoca S, Robu A, Neagu M, Neagu A, Mathematical and computational models in spheroid-based biofabrication. Acta Biomaterialia 2023, 165, 125-139. https:\/\/doi.org\/10.1016\/j.actbio.2022.07.024<\/a><\/li>\n<\/ol>\n
<\/p>\n
Participare la conferin\u021be<\/strong>:<\/p>\n
- \n
- Bojin F, Robu A, Bejenariu MI, Ordodi V, Olteanu E, Cean A, Popescu R, Neagu M, Gavriliuc O, Neagu A, Arjoca S, Paunescu V. \u201e3D bioprinting of model tissues that mimic the tumor microenvironment\u201d, poster, 13th European Biophysics Conference (EBSA 2021), 24-28 iulie 2021, Viena, Austria<\/li>\n
- Bojin F, Robu A, Bejenariu MI, Ordodi V, Olteanu E, Cean A, Popescu R, Neagu M, Gavriliuc O, Neagu A, Arjoca S, Paunescu V. \u201e3D bioprinting of model tissues that mimic the tumor microenvironment\u201d, poster, International Conference on Biofabrication, 27-29 septembrie 2021, online event, Wollongong, Australia<\/li>\n
- Bojin F, Robu A, Bejenariu MI, Ordodi V, Olteanu E, Cean A, Popescu R, Neagu M, Gavriliuc O, Neagu A, Arjoca S, Paunescu V. \u201e3D bioprinting of model tissues that mimic the tumor microenvironment\u201d, poster, 1st Oncohub Conference, 13-15 octombrie 2021, online event, Bucure\u0219ti, Rom\u00e2nia<\/li>\n
- Bojin F, Arjoca S, Bejenariu MI, Ordodi V, Olteanu E, Cean A, Popescu R, Neagu M, Gavriliuc O, Neagu A, Paunescu V. \u201eBiofabtication of tumor models that mimic the tumor microenvironment using extrusion bioprinting\u201d \u2013 prezentare oral\u0103, Tissue Engineering and Regenerative Medicine International Society (TERMIS) European Conference 2022, 26 iunie \u2013 1 iulie 2022, Cracovia, Polonia<\/li>\n
- Arjoca S, Robu A, Bojin F, Ordodi V, Olteanu E, Cean A, Neagu M, Gavriliuc O, Neagu A, Paunescu V. \u201ePost-printing structure formation in bioprinted tissue constructs that mimic the tumor microenvironment\u201d \u2013 prezentare oral\u0103, Tissue Engineering and Regenerative Medicine International Society (TERMIS) European Conference 2022, 26 iunie \u2013 1 iulie 2022, Cracovia, Polonia<\/li>\n
- Bojin F, Arjoca S, Bejenariu MI, Ordodi V, Olteanu E, Cean A, Popescu R, Neagu M, Gavriliuc O, Neagu A, Paunescu V. \u201eBiofabrication of breast cancer models using extrusion bioprinting\u201d \u2013 prezentare oral\u0103, 17th National Conference of Biophysics with International Participation (CNB2022), 23-25 septembrie 2022, T\u00e2rgu Mure\u0219, Rom\u00e2nia<\/li>\n
- Arjoca S, Robu A, Bojin F, Ordodi V, Olteanu E, Cean A, Neagu M, Gavriliuc O, Neagu A, Paunescu V. \u201eMulticellurar self-organization in bioprinted models of tumor microevironment\u201d \u2013 prezentare oral\u0103, 17th National Conference of Biophysics with International Participation (CNB2022), 23-25 septembrie 2022, T\u00e2rgu Mure\u0219, Rom\u00e2nia<\/li>\n<\/ol>\n
[\/et_pb_toggle][et_pb_toggle title=”Dezvoltarea de pachete software pentru biotip\u0103rirea unor vase de s\u00e2nge” open_toggle_text_color=”gcid-2dd42c1b-4a6d-4417-9dc5-a03359dc6473″ open_toggle_background_color=”#FFFFFF” closed_toggle_background_color=”gcid-2dd42c1b-4a6d-4417-9dc5-a03359dc6473″ icon_color=”#FFFFFF” open_icon_color=”gcid-2dd42c1b-4a6d-4417-9dc5-a03359dc6473″ _builder_version=”4.23.1″ _module_preset=”default” title_text_color=”#FFFFFF” title_level=”h4″ title_font=”|600|||||||” hover_enabled=”0″ border_radii=”on|15px|15px|15px|15px” border_style_all=”none” global_colors_info=”{%22gcid-2dd42c1b-4a6d-4417-9dc5-a03359dc6473%22:%91%22closed_toggle_background_color%22,%22open_toggle_text_color%22,%22open_icon_color%22,%22closed_toggle_background_color%22,%22open_toggle_text_color%22,%22open_icon_color%22%93}” sticky_enabled=”0″]<\/p>\n
Detalii<\/strong>:<\/p>\n
- \n
- Dezvoltarea de pachete software pentru biotip\u0103rirea unor vase de s\u00e2nge.<\/em> Limbajul Python a fost folosit pentru dezvoltarea softului \u0219i a interfe\u021bei grafice. Geometria vizat\u0103 este de tub helicoidal \u0219i poate fi u\u0219or scalabil\u0103.<\/li>\n
- Validarea experimental\u0103 a programelor dezvoltate.<\/em> Modele de vase de s\u00e2nge de diferite dimensiuni au fost tip\u0103rite cu succes individual sau in pl\u0103ci cu mai multe godeuri.<\/li>\n
- Evaluarea constructelor post-tip\u0103rire<\/em>. Modelele de vase de s\u00e2nge au fost evaluate histologic la diferite perioade de timp de cultura in vitro.<\/li>\n<\/ul>\n
![\"\"](\"https:\/\/oncogen.ro\/wp-content\/uploads\/2023\/12\/Figura_prototip_vase_de_sange-300x242.png\")
<\/p>\nPublica\u021bii<\/strong>: manuscris \u00een curs de finalizare \u0219i publicare.<\/p>\n
Particip\u0103ri la conferin\u021be<\/strong>:<\/p>\n
- \n
- Nistor A, Arjoca S, Bojin F, Zoric A, Salagean A, Paunescu V. \u201eBioengineering of implantable 3D vascular bioconstruct using rapid prototyping techniques\u201d, poster, 1st Oncohub Conference, 13-15 octombrie 2021, online event, Bucure\u0219ti, Rom\u00e2nia<\/li>\n<\/ol>\n
[\/et_pb_toggle][et_pb_toggle title=”Dezvoltarea de scripturi Python pentru evaluarea printabilit\u0103\u021bii hidrogelurilor \u00een procesele de biotip\u0103rire” open_toggle_text_color=”gcid-2dd42c1b-4a6d-4417-9dc5-a03359dc6473″ open_toggle_background_color=”#FFFFFF” closed_toggle_background_color=”gcid-2dd42c1b-4a6d-4417-9dc5-a03359dc6473″ icon_color=”#FFFFFF” open_icon_color=”gcid-2dd42c1b-4a6d-4417-9dc5-a03359dc6473″ _builder_version=”4.23.1″ _module_preset=”default” title_text_color=”#FFFFFF” title_level=”h4″ title_font=”|600|||||||” border_radii=”on|15px|15px|15px|15px” border_style_all=”none” global_colors_info=”{%22gcid-2dd42c1b-4a6d-4417-9dc5-a03359dc6473%22:%91%22closed_toggle_background_color%22,%22open_toggle_text_color%22,%22open_icon_color%22,%22closed_toggle_background_color%22,%22open_toggle_text_color%22,%22open_icon_color%22%93}”]<\/p>\n
Detalii<\/strong>:<\/p>\n
- \n
- Dezvoltarea de scripturi Python pentru generarea \u0219i tip\u0103rirea unor geometrii tridimensionale complexe<\/em>.<\/li>\n
- Testarea printabilit\u0103\u021bii unor hidrogeluri folosind modelele dezvoltate<\/em>. Constructele au fost analizate din punct de vedere al reproductibilit\u0103\u021bii \u0219i al factorilor geometrici (fidelitatea formei, deflexia filamentelor suspendate, etc.) sau de proces (viteza de extrudare, presiunea aplicat\u0103, diametrul filamentului depus, etc.).<\/li>\n<\/ul>\n
\u00a0<\/strong><\/p>\n
Publica\u021bii<\/strong>: manuscris \u00een curs de finalizare \u0219i publicare.<\/p>\n
[\/et_pb_toggle][\/et_pb_column][\/et_pb_row][et_pb_row disabled_on=”on|on|on” _builder_version=”4.23.1″ _module_preset=”default” disabled=”on” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.23″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.23″ _module_preset=”default” global_colors_info=”{}”]<\/p>\n
\u00cen cadrul proiectului INSPIRED (Strategii inovative pentru preven\u021bia, diagnosticul \u0219i terapia afec\u021biunilor respiratorii induse de polenul de ambrozia) s-a identificat profilul de sensibilizare IgE al pacien\u021bilor alegici la polenul de ambrozia (Ambrosia artemisiifolia<\/em>).<\/p>\n
[\/et_pb_text][et_pb_image src=”https:\/\/oncogen.ro\/wp-content\/uploads\/2023\/11\/poza-1.png” title_text=”poza 1″ _builder_version=”4.23″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_image][et_pb_text _builder_version=”4.23″ _module_preset=”default” global_colors_info=”{}”]<\/p>\n
Profil de sensibilizare identificat \u00een r\u00e2ndul pacien\u021bilor alergici la polenul de ambrozia conform Buzan et al. 2022<\/p>\n
Pe baza acestui profil au fost identificate \u0219i caracterizate alergenele din polenul de ambrozia (Ambrosia artemisiifolia<\/em>). Pentru a efectua caracterizarea, s-a identificat secven\u021ba proteic\u0103 a acestor alergene din bazele de date (allergen.org) \u0219i s-a realizat un construct pentru expresia acestora \u00een celule procariote, Escherichia coli<\/em>, \u0219i eucariote, Spodoptera frugiperda (Sf9)<\/em>.<\/p>\n
[\/et_pb_text][et_pb_image src=”https:\/\/oncogen.ro\/wp-content\/uploads\/2023\/11\/poza-2.jpg” title_text=”poza 2″ _builder_version=”4.23″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_image][et_pb_text _builder_version=”4.23″ _module_preset=”default” global_colors_info=”{}”]<\/p>\n
Model 3D a alergenelor din polenul de ambrozia ob\u021binute \u00een https:\/\/swissmodel.expasy.org\/<\/a><\/p>\n
Sistemul de expresie a fost ales \u00een func\u021bie de complexitatea structural\u0103 a proteinei.<\/p>\n
Constructul codific\u00e2nd proteina de interes a fost inserat \u00een genomul celulelor procariote prin administrarea unui \u0219oc termic. Apoi s-a ad\u0103ugat un factor de transcrip\u021bie pentru a induce expresia proteic\u0103. Pentru a produce proteinele \u00een celule de insecte, constructul genic a fost introdus \u00een celule E. coli<\/em> pentru amplificare \u0219i ob\u021binerea unui bacmid cu secven\u021ba de interes. Acesta a fost apoi folosit pentru transfec\u021bia celulelor de insecte, amplificarea stocului baculoviral \u0219i produc\u021bia de proteine. Proteinele au fost apoi izolate \u0219i purificate prin cromatografie de afinitate. Puritatea a fost verificat\u0103 pe SDS-PAGE. Produc\u021bia mai detaliat\u0103 a proteinelor se reg\u0103se\u0219te \u00een Zbircea et al. 2023, T\u0103ma\u0219 et al. 2023, Grijincu et al. 2023.<\/p>\n
[\/et_pb_text][et_pb_image src=”https:\/\/oncogen.ro\/wp-content\/uploads\/2023\/11\/poza-3.png” title_text=”poza 3″ align=”center” force_fullwidth=”on” _builder_version=”4.23″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_image][et_pb_text _builder_version=”4.23″ _module_preset=”default” global_colors_info=”{}”]<\/p>\n
Pa\u0219ii parcur\u0219i \u00een vederea producerii de alergene recombinate<\/p>\n
[\/et_pb_text][et_pb_image src=”https:\/\/oncogen.ro\/wp-content\/uploads\/2023\/11\/poza-4.png” title_text=”poza 4″ align=”center” _builder_version=”4.23″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_image][et_pb_text _builder_version=”4.23″ _module_preset=”default” global_colors_info=”{}”]<\/p>\n
Alergene recombinate din polenul de ambrozia (SDS-PAGE)<\/p>\n
Aceste proteine au fost incluse \u00eentr-un brevet pentru dezvoltarea unui kit de diagnostic al alergiei la polenul de ambrozia. Ele au fost folosite \u0219i pentru a ob\u021bine anticorpi alergen-specifici care s-a folosit \u00eentr-un al doilea brevet pentru detectarea alergenelor din polenul de ambrozia \u00een<\/p>\n
Brevete rezultate \u00een urma proiectului INSPIRED<\/strong><\/p>\n
Panaitescu C., Chen KW., P\u0103unescu V., Buzan RM.<\/p>\n
\u201eKit \u0219i metod\u0103 de utilizare pentru diagnosticul alergiei l la ambrozie\u201d, nr.A \/00615 \/2020, data de depozit 30\/09\/2020, Oficiul de Stat pentru Inven\u021bii \u0219i M\u0103rci (OSIM), Direc\u021bia de brevete de inven\u021bie \u0219i informa\u021bii tehnologice.<\/p>\n
Panaitescu C.,Chen KW., Buzan RM., Grijincu M., Zbircea LE.,Tamas TP., Haidar L.,Tanasie G., Hutu I.<\/p>\n
\u201eKit de testare cu anticorpi alergen-specifici pentru detec\u021bia \u0219i cuantificarea alergenelor din polenul de Ambrozia din mediu \u0219i din extracte alergenice diagnostice\/terapeutice\u201d,nr. A\/ 00759\/ 2020, data de depozit 19\/11\/2020,Oficiul de Stat pentru Inven\u021bii \u0219i M\u0103rci (OSIM), Direc\u021bia de brevete de inven\u021bie \u0219i informa\u021bii tehnologice.<\/p>\n
Publica\u021bii rezultate \u00een urma proiectului INSPIRED<\/strong><\/p>\n
Chen et al. 2018 – Ragweed Pollen Allergy: Burden, Characteristics, and Management of an Imported Allergen Source in Europe \u2013 Impact factor 2.932<\/strong> – https:\/\/karger.com\/iaa\/article\/176\/3-4\/163\/168362<\/a><\/p>\n
Panaitescu et al. 2022 – Precision medicine in the allergy clinic: the application of component resolved diagnosis – Impact Factor: 4.47<\/strong> – https:\/\/www.tandfonline.com\/doi\/full\/10.1080\/1744666X.2022.2034501<\/a><\/p>\n
Bumb\u0103cea et al. 2022 -Mechanisms of allergen immunotherapy supporting its disease-modifying effect – Impact Factor: 4.19<\/strong> – https:\/\/www.futuremedicine.com\/doi\/abs\/10.2217\/imt-2021-0325<\/a><\/p>\n
Marusciac et al. 2021 – Symptom patterns and comparison of diagnostic methods in ragweed pollen allergy \u2013 Impact factor 2.751<\/strong> – https:\/\/www.spandidos-publications.com\/10.3892\/etm.2021.9957<\/a><\/p>\n
Buzan et al. 2022 – Complex IgE sensitization patterns in ragweed allergic patients: Implications for diagnosis and specific immunotherapy – Impact Factor: 5.657<\/strong>– https:\/\/onlinelibrary.wiley.com\/doi\/full\/10.1002\/clt2.12179<\/a><\/p>\n
Zbircea et al. 2023 – Relationship between IgE Levels Specific for Ragweed Pollen Extract, Amb a 1 and Cross-Reactive Allergen Molecules \u2013 Impact factor 6.208<\/strong> – https:\/\/www.mdpi.com\/1422-0067\/24\/4\/4040<\/a><\/p>\n
Tama\u0219 et al. 2023 – Ragweed Major Allergen Amb a 11 Recombinant Production and Clinical Implications – Impact Factor: 6.064<\/strong> – https:\/\/www.mdpi.com\/2218-273X\/13\/1\/182<\/a><\/p>\n
Grijincu et al. 2023 – Physicochemical and immunological characterization of Amb a 12, a novel ragweed (Ambrosia artemisiifolia) pollen allergen \u2013 Impact factor 4.174<\/strong> – https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0161589023000548<\/a><\/p>\n
Rezultate acestei cercet\u0103ri au fost \u0219i vor fi incluse \u00een urm\u0103toarele teze de doctorat sub coordonarea doamnei prof. univ. dr. Panaitescu Carmen:<\/p>\n
<\/p>\n
\n\n
\n Nume Prenume<\/strong><\/td>\n Titlu tez\u0103<\/strong><\/td>\n<\/tr>\n \n Tama\u0219 Tudor Paul<\/td>\n Alergenul major Amb a 11 din polenul de ambrozia (Ambrosia artemisiifolia<\/em>): expresie in sistem procariot si eucariot, aspecte imunoproteomice \u0219i corelatii clinice<\/td>\n<\/tr>\n \n Buzan Maria Roxana<\/td>\n Caracterizarea imunoproteomic\u0103 a alergenelor recombinate din polenul de Ambrosia artemisiifolia<\/em><\/td>\n<\/tr>\n \n Zb\u00eercea Lauriana-Eunice<\/td>\n Evaluarea reac\u021biei de reactivitate \u00eencruci\u0219at\u0103 \u0219i testarea relevan\u021bei clinice a unor alergene din Ambrosia artemisiifolia<\/em><\/td>\n<\/tr>\n \n Grijincu Manuela<\/td>\n Caracterizarea unei noi proteine alergenice din familia liazelor din polenul de ambrozia (Ambrosia artemisiifolia<\/em>) \u0219i evaluarea relevan\u021bei clinice a acesteia<\/td>\n<\/tr>\n \n Zimbru Razvan<\/td>\n Evaluarea markerilor inflama\u021biei alergice prin metode noninvazive<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n [\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"
Transfer TehnologicDetalii: Dezvoltarea de pachete software pentru tip\u0103rirea de modele tumorale (toroidal \u0219i triplu stratificat). Programele au fost scrise \u00een limbajul Python, au interfa\u021b\u0103 grafic\u0103, \u0219i permit generarea de instruc\u021biuni de tip G-code cu geometrie scalabil\u0103. Validarea experimental\u0103 a programelor dezvoltate. Ambele tipuri de structuri, con\u021bin\u00e2nd celule tumorale \u0219i peritumorale, au fost tip\u0103rite individual sau […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","inline_featured_image":false,"footnotes":""},"class_list":["post-101702","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/oncogen.ro\/ro\/wp-json\/wp\/v2\/pages\/101702","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/oncogen.ro\/ro\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/oncogen.ro\/ro\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/oncogen.ro\/ro\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"http:\/\/oncogen.ro\/ro\/wp-json\/wp\/v2\/comments?post=101702"}],"version-history":[{"count":8,"href":"http:\/\/oncogen.ro\/ro\/wp-json\/wp\/v2\/pages\/101702\/revisions"}],"predecessor-version":[{"id":101938,"href":"http:\/\/oncogen.ro\/ro\/wp-json\/wp\/v2\/pages\/101702\/revisions\/101938"}],"wp:attachment":[{"href":"http:\/\/oncogen.ro\/ro\/wp-json\/wp\/v2\/media?parent=101702"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
- Testarea printabilit\u0103\u021bii unor hidrogeluri folosind modelele dezvoltate<\/em>. Constructele au fost analizate din punct de vedere al reproductibilit\u0103\u021bii \u0219i al factorilor geometrici (fidelitatea formei, deflexia filamentelor suspendate, etc.) sau de proces (viteza de extrudare, presiunea aplicat\u0103, diametrul filamentului depus, etc.).<\/li>\n<\/ul>\n
- Dezvoltarea de scripturi Python pentru generarea \u0219i tip\u0103rirea unor geometrii tridimensionale complexe<\/em>.<\/li>\n
- Validarea experimental\u0103 a programelor dezvoltate.<\/em> Modele de vase de s\u00e2nge de diferite dimensiuni au fost tip\u0103rite cu succes individual sau in pl\u0103ci cu mai multe godeuri.<\/li>\n
- Dezvoltarea de pachete software pentru biotip\u0103rirea unor vase de s\u00e2nge.<\/em> Limbajul Python a fost folosit pentru dezvoltarea softului \u0219i a interfe\u021bei grafice. Geometria vizat\u0103 este de tub helicoidal \u0219i poate fi u\u0219or scalabil\u0103.<\/li>\n
- Arjoca S, Robu A, Neagu M, Neagu A, Mathematical and computational models in spheroid-based biofabrication. Acta Biomaterialia 2023, 165, 125-139. https:\/\/doi.org\/10.1016\/j.actbio.2022.07.024<\/a><\/li>\n<\/ol>\n
- Validarea experimental\u0103 a programelor dezvoltate<\/em>. Ambele tipuri de structuri, con\u021bin\u00e2nd celule tumorale \u0219i peritumorale, au fost tip\u0103rite individual sau consecutiv \u00een pl\u0103ci cu mai multe godeuri.<\/li>\n