Molecular biology laboratory

Short presentation:
The lab is envisioned for molecular diagnostics in cancer. Molecular tests enable targeted cancer diagnosis as well as appropriate therapy selection. Molecular diagnostics, which focuses on how genes and proteins are interacting in a cell, allows the identification of expression patterns characteristic of a set of changes that can be used to identify cancer. These expression patterns can also be used as tools to improve the diagnosis. Interpretation of the expression levels or copy number variation of genes can provide valuable information relating to disease progression. Harnessing such gene expression profiles can be used to tailor or monitor therapies. The lab is equipped with high-fidelity devices, ensuring the most favorable PCR efficiencies needed for sensitive and specific quantification of a wide range of nucleic acid targets such as mRNA, microRNA and non-coding RNA.

Main activities/techniques:

  • sample preparation and nucleic acids extraction
  • gene expression and copy number variation (CNV) analysis
  • mutation detection; SNP genotyping
  • activities allowing generation of personalized treatment plans

Personnel:
Coordinator: Calin Tatu, MD, PhD
Oana Gavriliuc, Biol., MS, PhD
Florina Bojin, MD, PhD
Alexandra Ivan, Biotech Eng., PhD
Technicians

Equipment:

  • LightCycler® 480 II Block Kit, 384 (Roche)
  • Veriti® Thermal Cycler (Applied Biosystems)
  • NanoDrop 1000 Spectrophotometer (Thermo Scientific)
  • Molecular Imager®Gel Doc™ XR+ Universal Hood II (Bio-Rad)
  • Horizontal electrophoresis system Power Pack Mini-Sub® Cell GT Cell (Bio-Rad)
  • TissueLyser LT (Qiagen)
  • Thermoblock Eppendorf SmartBlock (Eppendorf)
  • Compact Thermomixers with thermoblock (Eppendorf)
  • Laboratory centrifuges SL40R (Thermo Scientific)
  • MiniSpin Centrifuge 5453 (Eppendorf)
  • Low temperature freezers (- 86o C) (Thermo Scientific)

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