Histology and Cellular Imaging Laboratory

Short Presentation:
The lab is design for investigating the biology and genetics of cancer and other diseases, developing and applying leading edge technology to diagnostic pathology, providing collaborative support for clinical research protocols. The lab provides a comprehensive range of histological research techniques that include: standard methods for inclusion in paraffin, special orientation of tissues, serial sectioning and sectioning of frozen specimens. In addition to the routine hematoxylin and eosin staining of paraffin sections, there are special stains to highlight specific cells, tissue components or other cellular elements. Special equipment is available for viewing slides and a photo system for telepathology is available for three-dimensional reconstruction of specimens.

Main activities/techniques:

  • preparation of histologic sections from both paraffin-embedded and frozen tissue, including serial sections
  • hematoxylin and eosin staining
  • special stains
  • immunohistochemical staining

Personnel:
Coordinator: Daciana Nistor, MD, PhD
Calin Tatu, MD, PhD
Alexandra Ivan, Biotech Eng., PhD
Technicians

Equipment:

  • Confocal microscope Carl Zeiss Axio Observer.Z1
  • Inverted microscope Carl Zeiss Axio Observer D1
  • Direct microscope Carl Zeiss Axio Scope A1
  • Microscope Hund with fluorescent lamp and photo system
  • Direct microscope Nikon E 600 with fluorescent lamp and photo system
  • Inverted microscope Olympus, with a digital system for capturing and storing images (Olympus photo camera, Olympus digital camera, VHS monitor, fluorescent lamp)
  • Cryotome Leica CM 1950
  • Microtome Leica RM 2255
  • Photo system for telepathology Carl Zeiss Axio Imager Z2

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